无机盐工业
主管:中海油天津化工研究设计院有限公司
主办:中海油天津化工研究设计院有限公司
   中海油炼油化工科学研究院(北京)有限公司
   中国化工学会无机酸碱盐专业委员会
ISSN 1006-4990 CN 12-1069/TQ

无机盐工业 ›› 2020, Vol. 52 ›› Issue (5): 18-21.doi: 10.11962/1006-4990.2019-0385

• 研究与开发 • 上一篇    下一篇

SERS基底Ag@Au的制备及其对氧氟沙星的检测

程昊1,2,徐娅娟1,2,李利军1,2,黄文艺1,2,卢浩1,2,冯军2,3()   

  1. 1.广西科技大学生物与化学工程学院,广西糖资源绿色加工重点实验室,广西柳州 545006;
    2.蔗糖产业省部共建协同创新中心;
    3.广西科技大学医学院
  • 收稿日期:2019-11-19 出版日期:2020-05-10 发布日期:2020-05-21
  • 通讯作者: 冯军
  • 作者简介:程昊(1980— ),男,副研究员,主要研究方向为应用化学;E-mail:1340509975@qq.com。
  • 基金资助:
    国家自然科学基金地区科学基金项目(31560466);广西高等学校高水平创新团队及卓越学者计划项目(桂教人[2014]7号);国家自然科学基金地区项目:功能化纳米复合材料hCEs SERS探针的构建及在血清与细胞中检测羧酸酯酶活性的应用研究(81860633);广西自然科学基金面上项目;微流控芯片-表面增强拉曼检测系统的构建及在单细胞分析中的应用(2016GXNSFAA380108);广西科技大学硕士研究生创新项目(GKYC201909)

Preparation of SERS substrate Ag@Au and its detection of ofloxacin

Cheng Hao1,2,Xu Yajuan1,2,Li Lijun1,2,Huang Wenyi1,2,Lu Hao1,2,Feng jun2,3()   

  1. 1.Guangxi Key Laboratory of Green Processing of Sugar Resources,College of Biological and Chemical Engineering,Guangxi University of Science and Technology,Liuzhou 545006,China;
    2.Province and Ministry Co-sponsored Collaborative Innovation Center of Sugarcane and Sugar Industry;
    3.School of Medicine,Guangxi University of Science and Technology
  • Received:2019-11-19 Online:2020-05-10 Published:2020-05-21
  • Contact: Feng jun

摘要:

通过种子介导生长方法,合成了形貌、粒径均一,分散性良好的双金属Ag@Au纳米粒子,并将其作为表面增强拉曼散射(SERS)基底,对氧氟沙星(OFLX)进行检测。首先在最佳实验条件下对氧氟沙星主要拉曼峰进行归属,选取1 416 cm -1拉曼特征峰。其次,对其1 416 cm -1特征峰强度与氧氟沙星浓度作线性拟合,曲线方程为Y=291.48X+3 156.8,r=0.989,检测极限可达10 -10 mol/L。该方法操作简单,灵敏度高,重现性好,可为氧氟沙星类抗生素药物的SERS检测提供依据。

关键词: SERS, Ag@AuNPs, 氧氟沙星

Abstract:

A bimetallic Ag@Au nanoparticle with uniform morphology,uniform particle size and good dispersibility was syn-thesized by seed-mediated growth method and used as a surface-enhanced Raman scattering(SERS) substrate for ofloxacin (OFLX) testing.Firstly,the main Raman peak of ofloxacin was assigned under the optimal experimental conditions,and the 1 416 cm -1 Raman characteristic peak was selected.Secondly,the characteristic peak intensity of 1 416 cm -1 was linearly fit-ted with the concentration of ofloxacin.The curve equation was Y=291.48X+3 156.8,r=0.989,and the detection limit reached 10 -10 mol/L.The method is simple in operation,high in sensitivity and good in reproducibility,and can provide a basis for SERS detection of ofloxacin antibiotic drugs.

Key words: SERS, Ag@AuNPs, ofloxacin

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