二氧化铈量子点纳米酶的制备及用于抗坏血酸比色检测的研究
收稿日期: 2024-06-12
网络出版日期: 2024-07-11
基金资助
上海海洋大学资助项目(A2-2006-00-200212)
Study on preparation of cerium dioxide quantum dot nanozymes and their application in colorimetric detection of ascorbic acid
Received date: 2024-06-12
Online published: 2024-07-11
天然酶模拟物纳米酶的形貌和尺寸对酶的催化活性有显著影响。为了提高CeO2作为纳米酶的底物催化活性并拓宽其应用范围，采用硝酸铈作为前驱体，柠檬酸作为结构导向物质，通过一锅水热法制备了结晶性良好、颗粒尺寸较小的CeO2量子点（CeO2 QDs）。CeO2 QDs的小尺寸效应和丰富表面缺陷能够暴露出更多的底物接触活性位点，从而赋予其类氧化酶活性。其中，活性最佳的CeO2 QDs纳米酶（CeO2 QDs-6），最大粒径分布为（4.0±0.6） nm，表现出优于天然酶的氧化酶动力学特性，米氏动力学常数低至0.165 mmol/L，最大反应速率可达9.11×10-8 mol/（L·s）。基于抗坏血酸的抗氧化活性，构建了一种快速、灵敏且高选择性的CeO2 QDs-6抗坏血酸比色检测探针。在优化的条件下，该探针在1.0~8.0 μmol/L线性范围内具有良好的抗坏血酸检测性能，最低检测限为148 nmol/L，并在牛奶样品检测中具有一定可行性。
唐淇 , 孙涛 , 薛斌 . 二氧化铈量子点纳米酶的制备及用于抗坏血酸比色检测的研究[J]. 无机盐工业, 2025 , 57(6) : 35 -42 . DOI: 10.19964/j.issn.1006-4990.2024-0335
The morphology and size of nanozymes significantly affect their mimetic enzyme catalytic activity.In order to improve the substrate catalytic activity of CeO2 as a nanozyme and broaden its application range，using cerium nitrate as the precursor and citric acid as the structure-directing substance，CeO2 quantum dots（CeO2 QDs） with good crystallinity and small particle size were prepared by one-pot hydrothermal method.The small size effect and rich surface defects of CeO2 QDs could expose more substrate contact active sites，thereby endowing it with oxidase-liked activity.The optimal CeO2 quantum dot nanozyme，CeO2 QDs-6，with the maximum particle size distribution at （4.0±0.6） nm，showed better oxidase enzyme kinetic characteristics than natural enzymes，with the Michaelis kinetic constant as low as 0.165 mmol/L and the maximum reaction rate up to 9.11×10-8 mol/（L·s）.Based on the antioxidant activity of ascorbic acid，a rapid，sensitive and highly selective CeO2 QDs-6 ascorbic acid colorimetric detection probe was constructed.Under the optimized conditions，this probe had good ascorbic acid detection performance in the linear range of 1.0~8.0 μmol/L，the lowest detection limit was 148 nmol/L，and showed certain feasibility in the detection of milk samples.
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