无机盐工业
主管:中海油天津化工研究设计院有限公司
主办:中海油天津化工研究设计院有限公司
   中海油炼油化工科学研究院(北京)有限公司
   中国化工学会无机酸碱盐专业委员会
ISSN 1006-4990 CN 12-1069/TQ

无机盐工业 ›› 2025, Vol. 57 ›› Issue (6): 35-42.doi: 10.19964/j.issn.1006-4990.2024-0335

• 研究与开发 • 上一篇    下一篇

二氧化铈量子点纳米酶的制备及用于抗坏血酸比色检测的研究

唐淇1(), 孙涛1,2,3, 薛斌1,2,3,4()   

  1. 1.上海海洋大学食品学院化学系,上海 201306
    2.农业部水产品贮藏保鲜质量安全风险评估实验室(上海),上海 201306
    3.食品科学与工程国家级实验教学示范中心(上海海洋大学),上海 201306
    4.临港新片区海洋生物医药科技创新型平台,上海 201306
  • 收稿日期:2024-06-12 出版日期:2025-06-10 发布日期:2024-07-11
  • 通讯作者: 薛斌,男,博士,副教授,主要研究方向为功能材料化学及应用;E-mail:bxue@shou.edu.cn
  • 作者简介:唐淇,女,硕士,主要研究方向为食品分析化学;E-mail:409732677@qq.com
  • 基金资助:
    上海海洋大学资助项目(A2-2006-00-200212)

Study on preparation of cerium dioxide quantum dot nanozymes and their application in colorimetric detection of ascorbic acid

TANG Qi1(), SUN Tao1,2,3, XUE Bin1,2,3,4()   

  1. 1. Department of Chemistry,College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China
    2. Laboratory of Quality and Safety Risk Assessment for Aquatic Product on Storage and Preservation(Shanghai),Ministry of Agriculture,Shanghai 201306,China
    3. National Experimental Teaching Demonstration Center for Food Science and Engineering(Shanghai Ocean University),Shanghai 201306,China
    4. Marine Biomedical Science and Technology Innovation Platform of Lingang Special Area,Shanghai 201306,China
  • Received:2024-06-12 Published:2025-06-10 Online:2024-07-11

摘要:

天然酶模拟物纳米酶的形貌和尺寸对酶的催化活性有显著影响为了提高CeO2作为纳米酶的底物催化活性并拓宽其应用范围,采用硝酸铈作为前驱体,柠檬酸作为结构导向物质,通过一锅水热法制备了结晶性良好、颗粒尺寸较小的CeO2量子点(CeO2 QDs)。CeO2 QDs的小尺寸效应和丰富表面缺陷能够暴露出更多的底物接触活性位点,从而赋予其类氧化酶活性。其中,活性最佳的CeO2 QDs纳米酶(CeO2 QDs-6),最大粒径分布为(4.0±0.6) nm,表现出优于天然酶的氧化酶动力学特性,米氏动力学常数低至0.165 mmol/L,最大反应速率可达9.11×10-8 mol/(L·s)。基于抗坏血酸的抗氧化活性,构建了一种快速、灵敏且高选择性的CeO2 QDs-6抗坏血酸比色检测探针。在优化的条件下,该探针在1.0~8.0 μmol/L线性范围内具有良好的抗坏血酸检测性能,最低检测限为148 nmol/L,并在牛奶样品检测中具有一定可行性。

关键词: 二氧化铈, 纳米酶, 类氧化酶活性, 比色分析, 抗坏血酸

Abstract:

The morphology and size of nanozymes significantly affect their mimetic enzyme catalytic activity.In order to improve the substrate catalytic activity of CeO2 as a nanozyme and broaden its application range,using cerium nitrate as the precursor and citric acid as the structure-directing substance,CeO2 quantum dots(CeO2 QDs) with good crystallinity and small particle size were prepared by one-pot hydrothermal method.The small size effect and rich surface defects of CeO2 QDs could expose more substrate contact active sites,thereby endowing it with oxidase-liked activity.The optimal CeO2 quantum dot nanozyme,CeO2 QDs-6,with the maximum particle size distribution at (4.0±0.6) nm,showed better oxidase enzyme kinetic characteristics than natural enzymes,with the Michaelis kinetic constant as low as 0.165 mmol/L and the maximum reaction rate up to 9.11×10-8 mol/(L·s).Based on the antioxidant activity of ascorbic acid,a rapid,sensitive and highly selective CeO2 QDs-6 ascorbic acid colorimetric detection probe was constructed.Under the optimized conditions,this probe had good ascorbic acid detection performance in the linear range of 1.0~8.0 μmol/L,the lowest detection limit was 148 nmol/L,and showed certain feasibility in the detection of milk samples.

Key words: cerium dioxide, nanozyme, oxidase-liked activity, colorimetric analysis, ascorbic acid

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