Abstract: The preparation methods of mesoporous material,such as template method,hydrothermal method,sol-gel method,solvothermal method,ultrasound chemical method,and reverse micelles method,were summarized.The application of cationic surfactant,anionic surfactant,non-ionic surfactant,mixed surfactant,and non-surfactant in mesoporous materials preparation was introduced while giving a reference to template method.At last,several typical morphologies were expounded,and application of mesoporous materials in catalytic,adsorption separation,preparation of sensor electrode materials,and separation-and-fixation of enzyme were also briefly introduced.